What is Siberian Ginseng? Siberian ginseng for Memory aid?Siberian Ginseng Extract.
Contents
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- Siberian Ginseng Root.
- Origin of Siberian Ginseng.
- Botanical Description of Siberian Ginseng.
- Historical Uses of Siberian Ginseng.
- Active Properties of Siberian Ginseng.
- Phytochemicals and Constituents of Siberian Ginseng.
- Adaptogens Boosting the Immune System.
- Athletes and Antibiotics.
- Toxicity,Cautions and Contra-Indications.
- How to Take It and Dosage of Siberian Ginseng.
- Quantitative determination of Siberian Ginseng.
- Research Update of Siberian Ginseng.
- Photo Gallery of Eleutherococcus senticosus.
Quantitative determination of Siberian Ginseng.:
An analytical sample of stock should be pulverized down to the particle size that passes through the 2mm sieve according to State Standard (GOST) 214-83.
About 1 gram (precise weight) of pulverized stock should be put into a 100ml cone flask to carry out fractional extraction sequentially by 20ml two times by 70% ethyl alcohol and two times by 95% alcohol. Each extraction should be done in a magnetic mixer heated at less than 50 for 1 hour. The extract should be filtered through a paper filter into a 100ml round-bottom flask for distillation. Alcohol should be distilled to dryness under vacuum in a rotor evaporator.
To the remains in the flask should be added 10ml of water and 10ml of carbon tetrachloride. The flask contents should be thoroughly mixed and quantitatively transferred to a 100ml separating funnel. The flask should be rinsed twice by carbon tetrachloride by 5ml and rinse content should be added to the separating funnel contents. Then into the flask should be added 10ml of the mixture: chloroform and 95% alcohol (5:1) and then left for 10 minutes.
In the separating funnel, the water phase should be rinsed by thrice extraction by carbon tetrachloride by 10ml.
After separation of carbon tetrachloride, to the separating funnel contents should be added 20ml (including 10ml from the distillation flask) of the mixture: chloroform and 95% alcohol (5:1) and then extract eleutherozides for 5 minutes. The lower layer should be filtered through a paper filter with 2 grams of waterless sodium sulfate into a 100ml measuring flask.
Extraction of eleutherozides in the separating flask should be repeated four times more sequentially 15ml, 15ml, 10ml, and 10ml of the same mixture, collecting them into the same flask. Flask solution volume should be brought to the mark with the mixture: chloroform and 95% alcohol (5:1, Solution A).
20ml of Solution A should be put into a 50ml measuring flask and the solution volume should be brought to the mark with the mixture: chloroform and 95% alcohol (5:1, Solution B). Optical density of Solution B should be measured by a spectrometer with 278nm wavelength in a cuvette with a layer 10mm thick.
The mixture: chloroform and 95% alcohol (5:1) should be used as a reference solution.
The content of the sum of eleutherozides in the terms of eleutherozide B and absolutely dry stock in percentage terms (X) should be computed from the formula:
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Reference:
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- 1.What is Siberian Ginseng? Siberian ginseng for Memory aid?Siberian Ginseng Extract.
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