Turmeric Root or Curcuma Root,Phytochemicals and Applications.

Contents

• Basic Botanical Data and Identification of Turmeric Root.Curcuma Root.
• Description of Turmeric Root.Curcuma Root.
• Origin of Curcuma root.
• Curcuma Root Appearance and characters.
• Powdered plant material of Curcuma Root.
• Chemical assays and Phytochemicals of Turmeric Root.Curcuma Root.
• Medicinal uses of Turmeric Root.Curcuma Root.
• Experimental pharmacology of Turmeric Root.Curcuma Root.
• Functions of Turmeric Root.Curcuma Root.
• Curcuma Root Suggestions and Administrations.
• Literature from Chinese historical sources:Curcuma Root.
• Modern Researches of Turmeric Root.Curcuma Root.
• Curcuma longa Common Application.
• Dose escalation of a curcuminoid formulation.
• Effect of Curcuma longa and Ocimum sanctum on myocardial apoptosis in experimentally induced myocardial ischemic-reperfusion injury.
• Quality control including chromatographic fingerprint profiling.
• Research Update:Curcumae Longae.Curcuma longa.

Quality control including chromatographic fingerprint profiling.:

The quality control in Thai Herbal Pharmacopoeia Vol.I.Identification

 By Test A: Extract 10 g of the sample, in powder, with 2 ml acetic anhydride, add a few drops sulfuric acid and observe under ultraviolet light(366 nm): the solution shows blood-red colour.

 By TLC: Carry out the test as described in the "Thin-layer Chromatography"(Appendix 3.1), using silica gel G as the coating substance and a mixture of 49 volumes of benzene, 49 volumes of chloroform and 2 volumes of ethanol as the mobile phase but allowing the solvent front to ascend 17 cm above the line of application. Apply separately to the plate, 5 ul of each of the following two solutions. Prepare solution (A) by placing 1 g of the sample, in powder, in a stoppered test-tube, adding 3 ml of methanol and shaking for a while. Set aside for 1 hour and filter. For solution (B) dissolve 1 mg of curcumin in 1 ml of methanol. After removal of the plate from the chromatographic chamber, allow it to dry, and exmine under ultraviolet light (366 nm), locating the spots. The chromatogram obtained with solution (A) shows a yellow-brown spot (hRf value 28 to 34), corresponding to the curcumin spot from solution (B). Other two yellow-brown spots correspound in hRf values to the spot numbers 2 and 3. Several spots of higther and lower hRf values are observed (Table 1); see also Fig, V (Appendix 3.1H). Spray the plate with a 10 per cent w/v solution of phosphomolybdic acid in ethanol and heat at 105 Deg for 5 minutes; the spot due to curcumin is orange, orange-brown, blue, blue, and blue, respectively. Other spots of different colours are observed.

 

 Water not more than 10.0 per cent w/w (Azeotropic Distillation Method, Appendix 4.12).

 Foreign matter not more than 2.0 per w/w (Appendix 7.2).

 Acid-insoluble ash not more than 1.0 per cent w/w(Appendix 7.6).

 Total ash not more than 8.0 per cent w/w(Appendix 7.7).

 Ethanol-soluble extractive not less than 10.0 per cent w/w(Appendix 7.12).

 Water-soluble extractive not less than 9.0 per cent w/w(Appendix 7.12).

 Volatile oil not less than 6.0 per cent v/w. Use 10 g in No.180 powder, accurately weighed. Use 100 ml of water as the distillation liquid and a 500 ml round-bottomed flask. Distil at a rate of 2 to 3 ml per minute for 5 hours. Use 2.0 ml of xylene in the graduated tube. Calculate the content of volatile oil with reference to the anhydrous substance.
 


 Assay:

 Curcuminoids content Not less than 5.0 per cent w/w of curcuminoids, calculated as cuecumin, when determined by the following method.

 Standard curcumin solution Dissolve about 2 mg of curcumin, accurately weighed, in sufficient methanol to produce 5.0 ml.

 Standard curcumin curve Transfer into five 10 ml volumetric flasks, 20, 40, 50, 60, and 80 ul, respectively,of standard curcumin solution, dilute to volume with methanol, and mix. Measure the absorbances of the standard solutions relative to the blank at 420 nm. Plot the readings and draw the curve of best fit.

 Procedure:

 Transfer 300 mg of turmeric powder accurately weighed into a 10 ml volumetric flask, add tetrahydrofuran to volume and mix. Set aside at room temperature for 24 hours with frequent shaking. Dilute 1.0 mi of this the clear supernatant liquid with methanol to produce 25.0 ml. Transfer 1.0 ml of this solution into a 50 ml volumetric flask, dilute to volume with methanol and mix well. Measure the absorbance of the sample solution, and by reference to the Standard curcumin curve, calculate the content of curcuminoids as curcumin in the sample.

 hRf Values of components in methanolic extract of the rhizome of Curcumin longa Linn.:

 HPLC-Chromatogram of Tumeric: Chromatographic conditions; column: Inertsil ODS-3, 5 microm, 4.6 mmI. D. x 15 cm, mobile phase: (A) 2%(v/v) acetic acid in water, (B) acetonitrile, gradient condition: 40% B for 15 min, 100%B in 25 min, flow-rate: 1 ml/min, d:

Reference:

• 1.Turmeric Root or Curcuma Root,Phytochemicals and Applications.

This article written and edited via herbalist of MDidea Extracts Professional. They run a range of online descriptions about this herb,including general information related and summarized updating discoveries from findings of professional scientisits this field related.Describe style aimed to form a useful detecting literature space where the intertwined threshold and related questions raise out and visualize themselves.

♣ last edit date:18th,Oct.2010.