Comfrey leaf,Information About Comfrey leaf Extract.

Contents

• Basic Botanical Info:Symphytum officinale.
• Botanical Description:Symphytum officinale.
• Information About Comfrey leaf Extract.
• Comfrey Phytochemicals and Constituents:
• Medicinal uses:Comfrey Leaf.
• Actions and Indications:Comfrey Leaf.
• Comfrey Leaf Suggestions and Administrations.
• A rapid cleanup method for the isolation and concentration of pyrrolizidine alkaloids in comfrey root.
• Research Update:Comfrey leaf or Symphytum officinale.

A rapid cleanup method for the isolation and concentration of pyrrolizidine alkaloids in comfrey root.:

Preparations from comfrey (Symphytum officinale and S. x uplandicum) root and leaf contain varying levels of the hepatotoxic pyrrolizidine alkaloids (PAs). Reference compounds for comfrey are not commercially available, and there is currently no rapid extraction or analytical method capable of determining low levels in raw materials or as adulterants in commercially available extracts.

 A solid-phase extraction (SPE) method was developed using an Ergosil cleanup column that specifically binds the PAs. With this method, powdered comfrey root was extracted by sonication and shaking with basic chloroform. The extract was applied to the cleanup column under vacuum, washed with 2 mL acetone-chloroform (8 + 2, v/v) followed by 2 mL petroleum ether to remove excess chloroform. The column was dried under vacuum, and the PAs were eluted with 2 successive 1 mL aliquots methanol. Percent recoveries of the PAs following Ergosil SPE had an overall average of 96.8%, with RSD of 3.8% over a range of 1.0 to 25.0 g extracted in 100 mL. Average precision of the method (n = 3 over 4 extraction concentrations) gave an overall RSD of 6.0% for the 5 alkaloids, with a range of 0.8% (5 g in 100 mL) to 11.2% (25 g in 100 mL).

 Recovery optimization testing showed that 1.0 g comfrey root extracted in 100 mL yielded the greatest recovery (% dry weight) of the PAs, with an extraction efficiency and accuracy of 94.2%,and RSD of 1.7% (n = 9).

 The unique properties of the Ergosil cleanup column provide rapid sample cleanup, volume reduction, and concentration of PAs from comfrey extracts, and allow the eluant to be analyzed directly by traditional chromatographic methods.

Reference:

• 1.Comfrey leaf,Information About Comfrey leaf Extract.

This article written and edited via herbalist of MDidea Extracts Professional. They run a range of online descriptions about this herb,including general information related and summarized updating discoveries from findings of professional scientisits this field related.Describe style aimed to form a useful detecting literature space where the intertwined threshold and related questions raise out and visualize themselves.

♣ last edit date:22nd,Oct.2010.